phosphogluconate dehydrogenase meaning in English

磷酸葡糖酸脱氢酶

Examples

It ' s pi is 5 . 2 as determined with ief . amino acid composition analysis showed that one subunit of 6 - phosphogluconate dehydrogenase has about 480 amino acids and there are plentiful of ala , asp , leu , ser , glu , thr , phe , lys in it
氨基酸组成分析表明：枯草芽孢杆菌6 -磷酸葡萄糖酸脱氢酶的亚基由约480个氨基酸残基组成，富含丙氨酸、门冬氨酸、亮氨酸、丝氨酸、谷氨酸、苏氨酸、苯丙氨酸、赖氨酸。
6 - phosphogluconate dehydrogenase ( 6 - pgadase , ec 1 . 1 . 1 . 44 ) was isolated by homogenate , ammunium sulfate fractionation , deae - sepharose chromatography , blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis , and some properties of the enzyme had been studied . a 113 . 8 - fold purification was obtained with a 8 . 2 % yield . the purified enzyme moved as a single electrophoretic band in page
将枯草芽孢杆菌超声波破壁后的粗提取物进行分段盐析、 deae - sepharose阴离子交换柱层析， blue - sepharosecl - 6b特异结合柱层析和sephadexg - 200凝胶过滤等纯化步骤，得到聚丙烯酰胺凝胶电泳为单一蛋白区带，比活为1 . 46u mg的酶制剂。
Kinetic study shows that nadp + is the only valid coenzyme and that one substrate have no effect on the the other substrates " bind with the enzyme . products are competitive inhibitors , nadp + / nadph and 6 - phosphogluconate / ribulose - 5 - phosphate pairs are competitive whatever the concentration of the other substrates but noncompetitive versus the other substrates . therefore the kinetic mechanism of the 6 - phosphogluconate dehydrogenase is haldance - laidler - socquet random mechanism
两种底物与酶的结合互不影响，而在产物抑制效果上， 5一磷酸核酮糖只竞争性抑制6一磷酸葡萄糖酸， nadph只竞争性抑制nadp + ，所以该酶催化机理是haidance一laidier一socquet随机机理。

phosphogluconate dehydrogenase meaning in English

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